Synthesis of DEAE-Soybean Starch Microspheres for Adhere Animal Cell Culture

Preparation of DEAE–soybean starch microspheres for anchorage-dependent mammal cell culture

The present study outlines the synthesis of a new microcarrier for anchoragedependent animal cell cultures similar to Cytodex 1. The new microcarriers were synthesized from the cross-linking soybean starch microspheres followed by modification with 2-diethylaminoethyl (DEAE). DEAE–soybean starch microspheres were used in anchorage-dependent cell cultures using 3 g/L of the carrier and a 2.0 × 1...

Toward Animal Cell Culture

Synthesis and Turnover of Cell-Wall Polysaccharides and Starch in Photosynthetic Soybean Suspension Cultures.

Soybean (Glycine max [L.] Merr.) suspension cultures grown under photoautotrophic and photomixotrophic (1% sucrose) culture conditions were used in 14CO2 pulse-chase experiments to follow cell-wall polysaccharide and starch biosynthesis and turnover. Following a 30-min pulse with 14CO2, about one-fourth of the 14C of the photoautotrophic cells was incorporated into the cell wall; this increased...

Development of Biodegradable Starch Microspheres for Intranasal Delivery

Domperidone microspheres for intranasal administration were prepared by emulsification crosslinking technique. Starch a biodegradable polymer was used in preparation of microspheres using epichlorhydrine as cross-linking agent. The formulation variables were drug concentration and polymer concentration and batch of drug free microsphere was prepared for comparisons. All the formulations were ev...

Carboxymethyl starch/alginate microspheres containing diamine oxidase for intestinal targeting

The association of carboxymethyl starch (CMS) and alginate is proposed as a novel matrix for the entrapment of bioactive agents in microspheres affording their protection against gastrointestinal degradation. In this study, the enzyme diamine oxidase (DAO) from white pea (Lathyrus sativus) was immobilized by inclusion in microspheres formed by ionotropic gelation of CMS/alginate by complexation...